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pet21 vectors  (GenScript corporation)
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Pet21 Vectors, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet21 6his 2nls vector  (Addgene inc)
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pet21(+) expression vector  (Merck & Co)
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Merck & Co pet21(+) expression vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21(+) Expression Vector, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet21(+) expression vector - by Bioz Stars, 2026-05
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pet21 vector  (New England Biolabs)
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New England Biolabs pet21 vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet21-b(+) expression vectors  (GenScript corporation)
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A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 B(+) Expression Vectors, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet21e vector  (Millipore)
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Millipore pet21e vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21e Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet21(+) vector (ampicillin  (Twist Bioscience)
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Twist Bioscience pet21(+) vector (ampicillin
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
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pet21 vector  (Millipore)
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A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet21 mruby2 vector  (New England Biolabs)
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New England Biolabs pet21 mruby2 vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 Mruby2 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pet21-pelb e. coli periplasmic expression vector  (Agilent technologies)
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Agilent technologies pet21-pelb e. coli periplasmic expression vector
A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 <t>pET21_</t> fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).
Pet21 Pelb E. Coli Periplasmic Expression Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

Journal: bioRxiv

Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

doi: 10.1101/2024.12.02.626473

Figure Lengend Snippet: A) Representative 0.3% swim plate (120 mm) of all FliC chimeras. E. coli Δ motAB fliC :: tetRA (SYC29) pDB108 pET21_ fliC and E. coli SYC29 pDB108 pET21 were used as positive and negative controls, respectively. Plates were incubated at 30°C for 16 hrs. B) Quantification of the mean swim ring diameter of each chimeric and outer domain deleted FliC recorded from three independent 0.3% swim plates incubated at 30°C for 16 hrs. Error bars indicate standard error of the mean. A two-way ANOVA comparing each column to the FliC value was used to generate the p values (** = ≤ 0.01, *** = ≤ 0.001).

Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

Techniques: Incubation

SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

Journal: bioRxiv

Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

doi: 10.1101/2024.12.02.626473

Figure Lengend Snippet: SDS-PAGE gel of non-motile FliC chimera constructs (ECHM and ECCF) heat treated supernatants. Empty vector pET21 and pET21_ fliC were used as a negative and positive control, respectively. BSA was used as a marker control.

Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

Techniques: SDS Page, Construct, Plasmid Preparation, Positive Control, Marker, Control

Journal: bioRxiv

Article Title: Rescue of bacterial motility using two and three-species FliC chimeras

doi: 10.1101/2024.12.02.626473

Figure Lengend Snippet:

Article Snippet: Digestion of gene fragments and pET21(+) (Merck) expression vector was performed using EcoRI-HF and NotI-HF (NEB), followed by Antarctic phosphatase (NEB) treatment of pET21(+), as per the manufacturer’s instructions.

Techniques: